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Science/Tech
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Title: Why are Chesapeake rockfish sick?
Source: Delawareonline.com
URL Source: http://www.delawareonline.com/apps/ ... 70610/NEWS/706100385/1006/NEWS
Published: Jun 10, 2007
Author: By KRISTEN WYATT, Associated Press
Post Date: 2007-06-10 08:37:41 by HOUNDDAWG
Keywords: chronic, wasting, disease
Views: 609
Comments: 54

Rockfish with the disease mycobacteriosis can develop lesions and have their scales destroyed. AP/KATHLEEN LANGE

OXFORD, Md. -- Biologist Larry Pieper is wearing waterproof overalls and giving dozens of bass from the Chesapeake Bay a tabletop inspection. He's not surprised by what he sees -- many of them look sick.

Pieper is part of Maryland's first effort this year to take a new look at a chronic wasting disease in striped bass, commonly called rockfish or stripers.

Mycobacteriosis, also known as "myco" or fish handler's disease, can slowly eat away at a fish's scales. It can leave nasty lesions and kill the striped bass, the hallmark fish of the Chesapeake Bay. Beyond that, scientists don't know enough about the disease to know how worried they should be.

"We know a whole lot of nothing," Pieper said after examining 80 striped bass, noting lesions and little brown specks that suggest the bacteria are present.

Myco is usually harmless to humans, as long as anglers wash their hands after handling infected fish. However, scientists are startled by the bacteria's spread through the region. State biologists in Maryland and Virginia say that about 60 percent of the bass in the Chesapeake, where most of the East Coast's striped bass come to spawn, are now infected.

But scientists aren't entirely sure whether myco kills all striped bass that get it, or whether some striped bass recover, and why. It's not clear how myco spreads, nor why the disease has increased sharply in recent years.

It could be that there are simply more bass in the bay. After overharvesting nearly wiped out the striped bass, a fishing ban in the 1980s led to a remarkable rebound in the species, with fishing now allowed. Another suggestion is that more bass are getting sick because they're weakened by low food supplies, or because low oxygen levels in cold water drive them to warmer water, where they're more vulnerable to disease.

"When we started, there was lots of anecdotal information, lots of impressions, but nobody knows" exactly how the disease behaves or how it affects the fish, said John Hoenig, a researcher with the Virginia Institute of Marine Science in Gloucester Point, Va.

Bounty hunters

To find answers about the bacteria, biologists are trying a new method to learn about it. Maryland's Department of Natural Resources, along with a federal research group, started a new kind of tagging project this spring.

The research differs from traditional tagging because anglers are asked not just to call the number on the tag and report where and when they caught the fish, but to keep the fish on ice. Fishermen who do so get a $20 bounty, plus authorities will come pick up the fish.

On a tagging trip last week, 11 Maryland biologists tagged and examined 80 fish. Pieper looked at each fish and announced its condition to a notetaker -- "The left side is clear," he called, or, "We have healing on the right-side fin." Then the fish went to a white piece of wood for several photographs on each side, plus close-up shots of any lesions.

After a few moments on board, the tagged fish were tossed back into the Chesapeake.

Scientists hope that when anglers catch the tagged fish months or years from now, they'll be able to match the fish to their original pictures.

"Some of the fish that didn't look so good, they may come back in a year and be healed. We just don't know yet," Pieper said.

Economic impact

Virginia has been doing this type of work for two years, tagging 6,700 bass since 2005, but this is Maryland's first year joining the effort.

The research could yield lucrative findings. Recreational fishing in Maryland is an industry valued at more than $300 million a year, by state estimates. Unsightly lesions on striped bass and public worries about catching fish handler's disease can harm charter boat captains and commercial fishermen who depend on the fish for their livelihoods.

The research could prompt fishery managers to cap harvests or make other arrangements to help the population thrive despite the bacteria.

It's a new approach for officials used to considering only catch data when surveying a fish population. "Historically in fisheries, fishing is the problem," Hoenig said. When disease hits, he added, "We're sort of unprepared for that."

Figuring out clues

DNR biologist Kevin Rosemary, photographer for the striped bass, said that the research could prove valuable even if most fish don't get caught and called in. Dead fish are usually gobbled up quickly by other critters, so if the returned fish in Maryland's study were more likely to be disease-free when tagged, that could mean the sick fish were more likely to die. "We're hoping to learn if fish die of myco or not," Rosemary said.

He said Maryland hopes to tag 1,000 striped bass this year, some in the spring and then again in the fall.

Already the effort has yielded some clues. Hoenig said that when Virginia started its tagging, some people were critical because myco is always fatal in captive fish. Turns out, striped bass in the wild can live years with the bacteria, some plump and alert despite being mottled with lesions.

"Some people said it was silly because we're going to tag them, and then they're all going to die," Hoenig said. Now, as scientists learn more about how the bacteria affects the beloved striped bass, the next step could be clues for how to deal with mycobacteriosis.

"It's hard to convince people to take action if we can't demonstrate that there's problem," he said.

WANTED: STRIPED BASS WEARING GREEN TAGS

WANTED: Striped bass, also called rockfish or stripers, with a small green tag to report the catch.

THE CRIME: Scientists want to know more about mycobacteriosis, a mysterious wasting disease that now infects more than half the Chesapeake Bay's striped bass. Not all the tagged fish have the bacteria. But healthy fish are wanted, too, to see if they've stayed healthy since Maryland biologists tagged them or if they had the bacteria but recovered.

THE EVIDENCE: Some striped bass with the bacteria have mottled scales, or even nasty lesions. Some infected fish have tiny brown dots on some scales. Some infected fish show no outer signs of disease. The bacteria is usually harmless to humans, and if your poor hand-washing skills lead to an infection, the disease is easily treated with antibiotics. In rare cases of untreated mycobacteriosis, arthritis-like symptoms can result.

THE REWARD: Anglers get $5 for calling in a catch, or $20 if they keep the fish on ice for scientists to retrieve.

Source: Maryland Department of Natural Resources


Poster Comment:

When I was a kid my dad would wake my brother and I in the middle of the night so we could be on the fishing pier at Ocean View before sunrise. This pier at the mouth of the Chesapeake bay is one of my fondest memories because it wasn't unusual to catch 100 pan sized spot and croaker, give 60 away and take 40 or so home for a breakfast fish fry.

If you've never eaten a fresh (never frozen) ocean fish then you're letting the best of life pass you by.

But, in the mid 1970's a corporate type was convicted of the crime of dumping Kepone (a poison) into the James River which then proceeded to poison the fin and shellfish in the bay and leading to a years-long moratorium on their harvest. This was the first time someone went to jail for an environmental crime, and the impact on the bay and fishing was immediately obvious. Gone were the days of pulling up young pan fish two at a time, and my soul and the bay are forever damaged.

They still monitor the waters near the bridge tunnel (connecting Hampton and Norfolk) because of the very real threat that hurricanes may release sequestered Kepone.

The mighty striped bass is also a favorite fish here in DE river, where the migration alternates with the Chesapeake bay depending on unknown factors that determine the fish's choice of routes every spawning season. The Chesapeake bay and the Delaware river are connected by the C&D canal on the north end of the bay, so a problem with Chesapeake bay rockfish is a problem with Delaware rockfish.

What I wouldn't give to have lived when the Dutch landed here and the English landed in Jamestown, when clams were so thick that one could rake them from the clear waters all day every day non stop.

My soul is in agony when I read these stories. Words fail me beyond a mere account of the evidence of a crime for which there is no suitable punishment.

And, when Native Americans say that we whites have destroyed the land I have no answer....

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#15. To: JCHarris (#12)

I don't want to shoot from the hip so, I just say this for now.

The delicate estuaries of the bay still produce an abundance of blue crabs, and the fishing on the Susquehanna Flats is still wonderful.

When has a red tide or algae bloom resulted in a Chesapeake bay fish kill?

HOUNDDAWG  posted on  2007-06-10   15:01:14 ET  Reply   Trace   Private Reply  


#16. To: HOUNDDAWG (#15)

When has a red tide or algae bloom resulted in a Chesapeake bay fish kill?-Houndawg

"all the time" - me

Bay's 'dead zone' is back Pamela Wood - The Capital John Page Williams of the Chesapeake Bay Foundation checks oxygen levels in the Severn River Friday, finding low levels in deep waters. Lack of oxygen kills animals in water

By PAMELA WOOD, Staff Writer Once again, this summer could be a tough one for the Chesapeake Bay's critters. There are indications that the oxygen-deprived "dead zone" is starting to make its annual appearance.

While it's not surprising, it's yet another bad sign for the bay.

"All living resources - crabs, fish, oysters, clams - they need oxygen to survive," said Bruce Michael of the Maryland Department of Natural Resources.

When there's not enough oxygen, crabs and fish scuttle and look for better places to live. They can become susceptible to illness.

And critters that live at the bottom and can't move, such as oysters, can die from lack of oxygen.

Bobbing on his Boston Whaler in the middle of the Severn River yesterday afternoon, John Page Williams of the Chesapeake Bay Foundation could see the effects of the dead zone clear as day on his fish finder.

From the surface down to 10 feet, there were plenty of little squiggles indicating fish.

Below that - almost no fish.

Mr. Williams then dropped a handheld meter in the water. At approximately 13 feet, the meter read an oxygen level of 3 mg/liter. While that's not great, that's enough oxygen for shellfish and some fish. Ideal readings are 5 mg/liter or greater.

Then at nearly 20 feet, the meter read 0.6 mg/liter - a level of oxygen so low that almost nothing can survive.

"That's bad," he said.

Mr. Williams said he thinks its a bit early in the year to get such readings. He thinks the oxygen-deprived zone is shallower than in years past, too.

The Severn Riverkeeper Program's water monitoring crew is seeing similar numbers.

Riverkeeper intern Annelies Jane de Groot said there are "disappointingly low" oxygen levels around the entire river.

"It seems that oxygen is not permeating the Severn below 4 meters in depth," she said. Four meters is roughly 13 feet.

It's a similar case on the South River, where riverkeeper Drew Koslow said that seven of his 14 monitoring sites had readings below 5 mg/liter. At this time last year, five of his sites were that low.

Further south, the readings aren't encouraging in the West and Rhode rivers either, according to riverkeeper Bob Gallagher.

While the open areas of the rivers are doing well, the oxygen levels in the creeks and near shore are poor.

"We're seeing readings that seem to be lower than recent years," he said. "It's a little surprising to see this early in the year."

A broader monitoring program is finding evidence of the dead zone out in the open bay as well. "This is the time of year we do experience low dissolved oxygen," said Mr. Michael of the DNR.

Two baywide monitoring cruises in May showed low oxygen areas starting around the Bay Bridge, Mr. Michael said.

As the summer goes on, it's likely to get worse, he said.

"If it stays hot and sunny, this sets up and stays anoxic during the summer," he said.

Later this month, officials will unveil a summer forecast for the bay that will call for an average dead zone. The forecast is put out annually by the federal-state Chesapeake Bay Program, the DNR and the University of Maryland.

While the dead zone might come out as average, that's not good enough for Mr. Williams and the Chesapeake Bay Foundation.

CBF wants more focus on reducing the harmful nutrients that flow into the bay and cause the algae blooms that suck oxygen from the water.

"Is it better or worse? I don't give a damn. It's just bad," Mr. Williams said as he turned the Whaler back toward shore.

"Even if it's average," he added, "average stinks."

JCHarris  posted on  2007-06-10   15:06:39 ET  Reply   Trace   Private Reply  


#17. To: HOUNDDAWG (#15)

The delicate estuaries of the bay still produce an abundance of blue crabs, and the fishing on the Susquehanna Flats is still wonderful.

" While the open areas of the rivers are doing well, the oxygen levels in the creeks and near shore are poor."

JCHarris  posted on  2007-06-10   15:07:40 ET  Reply   Trace   Private Reply  


#18. To: JCHarris (#8)

The guts to change are lacking in the politicians who know what I say here is true and have not and will not do anything constructive about it

Amen!


It's not Global Warming, it's Ice Age Abatement.

farmfriend  posted on  2007-06-10   15:10:32 ET  Reply   Trace   Private Reply  


#19. To: HOUNDDAWG, farmfriend (#15)

Note the date....

and....

"benthic decay of organic detritus accumulated from plankton blooms of the previous summer and fall"

benthic is bottom

Science 6 January 1984: Vol. 223. no. 4631, pp. 22 - 27 DOI: 10.1126/science.223.4631.22

Chesapeake Bay Anoxia: Origin, Development, and Significance Charles B. Officer 1, Robert B. Biggs 2, Jay L. Taft 3, L. Eugene Cronin 4, Mary A. Tyler 5, and Walter R. Boynton 6

1 Research professor in the Department of Earth Sciences, Dartmouth College, Hanover, New Hampshire 03755. 2 Associate professor at the College of Marine Studies, University of Delaware, Newark 19711. 3 Director of administration at Harvard University Herbaria, Harvard University, Cambridge, Massachusetts 02138. 4 Director of Chesapeake Research Consortium, Shady Side, Maryland 20764. 5 Assistant professor at the College of Marine Studies, University of Delaware, Newark. 6 Associate professor at Chesapeake Biological Laboratories, University of Maryland, Solomons 20688.

Anoxia occurs annually in deeper waters of the central portion of the Chesapeake Bay and presently extends from Baltimore to the mouth of the Potomac estuary. This condition, which encompasses some 5 billion cubic meters of water and lasts from May to September, is the result of increased stratification of the water column in early spring, with consequent curtailment of reoxygenation of the bottom waters across the halocline, and benthic decay of organic detritus accumulated from plankton blooms of the previous summer and fall. The Chesapeake Bay anoxia appears to have had significant ecological effects on many marine species, including several of economic importance.

JCHarris  posted on  2007-06-10   15:19:43 ET  Reply   Trace   Private Reply  


#20. To: HOUNDDAWG (#15)

When has a red tide or algae bloom resulted in a Chesapeake bay fish kill?

all the time

BTW the process is called eutrophication . Wiki may have a section on this. I have not looked. You may well find it very interesting.

JCHarris  posted on  2007-06-10   15:27:53 ET  Reply   Trace   Private Reply  


#21. To: HOUNDDAWG (#0)

If you've never eaten a fresh (never frozen) ocean fish then you're letting the best of life pass you by.

I'm in a realllllly generous mood today, dawgie...........you can have every fishie in the seas/oceans/rivers/brooks/creeks/criks/bayous/fish tank that could conceivably have my name written on it! Yuck.........

That said, I do like tuna sandwiches with lots of 'stuff' in it. :)

rowdee  posted on  2007-06-10   15:44:22 ET  Reply   Trace   Private Reply  


#22. To: HOUNDDAWG (#5)

God keeps track of this stuff, don't kid yourself........'pissin' in His universe is not wise, IMO.....stewards, and we all are, are responsible for what we do.

rowdee  posted on  2007-06-10   15:48:00 ET  Reply   Trace   Private Reply  


#23. To: HOUNDDAWG (#5)

One mile away is the Christina river (named after the Queen of Sweden) and around 1900 the industrial dumping began there, and it is now a major SUPERFUND SITE.

IIRC, there was a Swedish colony in the Delaware area, mid 1600s...would have to look back thru my genealogy notes. I have an ancestor I'm trying to find who was born in Delaware and moved to NY, and came across that bit of info. I think most people have no idea regarding that bit of history.

rowdee  posted on  2007-06-10   15:50:53 ET  Reply   Trace   Private Reply  


#24. To: HOUNDDAWG, lodwick (#7)

If we someday find ourselves surrounded by bands of mutant cannibals who glow in the dark, the govt will tell the survivors that it was caused by environmentalists who insisted on mulching and recycling, and the people will believe it....

Awe, c'mon, DAWGIE......you can do better than that.

Like this: If we someday find ourselves surrounded by bands of mutant cannibals who glow in the dark, the govt will tell the survivors that it was caused by environmentalists who insisted on mulching and recycling terrorists who hate us for our freedoms, and the people will believe it....

rowdee  posted on  2007-06-10   15:53:21 ET  Reply   Trace   Private Reply  


#25. To: JCHarris (#8)

Pieper is part of Maryland's first effort this year to take a new look at a chronic wasting disease in striped bass, commonly called rockfish or stripers.

Looks like there's more evidence here of spiroplamas in the environment that weren't there before. How do you think they got there?


You appear to be a major trouble maker...and I'm getting really pissed. - GoldiLox, 7/27/2006

FormerLurker  posted on  2007-06-10   15:53:54 ET  Reply   Trace   Private Reply  


#26. To: FormerLurker (#25)

I haven't a clue they are there.

JCHarris  posted on  2007-06-10   15:58:34 ET  Reply   Trace   Private Reply  


#27. To: HOUNDDAWG (#15)

If you've never eaten a fresh (never frozen) ocean fish then you're letting the best of life pass you by.

Cobia. 35 lbs. Right out of the Atlantic 100 miles out of Wilmington NC; started real charcoal before I even prepared the fish, hosed down the boat at our townhouse and called a fvew more friends to join in;

two inch thick steaks, pure white ,

only basted with Italian dressing and quick grilled just to flakiness.

Corn on the cob, yeast rolls, tossed baby greens, lightly steamed summer squash and a fabulous Ferrari Fume' Blanc One dude brought a fresh churning oak tub of homemade real lemons ice cream. HOUNDAWG you should have not brought up that recollection.

JCHarris  posted on  2007-06-10   16:08:58 ET  Reply   Trace   Private Reply  


#28. To: JCHarris (#19)

Anoxia occurs annually in deeper waters of the central portion of the Chesapeake Bay and presently extends from Baltimore to the mouth of the Potomac estuary.

That trench off Annapolis is 100 feet deep, and nothing can live there.

But, fishers won't allow dredge spoils to be dumped there.

According to the Army Corps of Engineers Environmental Impact Report on the proposed deepening and widening of the channels, this trench has always been a dead zone.

So, what evidence have you that this is anything other than the normal cycle caused by the normal "decay of organic detritus accumulated from plankton blooms of the previous summer and fall"?

HOUNDDAWG  posted on  2007-06-10   16:10:19 ET  Reply   Trace   Private Reply  


#29. To: HOUNDDAWG (#28)

give me a break...give it up...just look at the harvests of anything you want to look up...that alone tells the story.

JCHarris  posted on  2007-06-10   16:11:57 ET  Reply   Trace   Private Reply  


#30. To: JCHarris (#8)

from Pennsylvania uneducated

JC...

Uneducated????

One should also scan the HUMAN pressure on Chesapeake Bay, those being by the MILLIONS living on and near the Bay.

Former MD resident.

Cynicom  posted on  2007-06-10   16:37:51 ET  Reply   Trace   Private Reply  


#31. To: JCHarris (#27)

When visiting my uncle in Oregon (early 70s) we had some salmon, fresh caught, grilled like steaks. I've never had the like again.

DeaconBenjamin  posted on  2007-06-10   16:45:14 ET  Reply   Trace   Private Reply  


#32. To: Cynicom (#30)

One should also scan the HUMAN pressure on Chesapeake Bay, those being by the MILLIONS living on and near the Bay.

Former MD resident.

Cynicom

That has been and is constantly done and still the vast majority of pollutants come from the Amish farmland and are transported by the Susquehanna with the others from the James to the Patuxent adding to the load.

JCHarris  posted on  2007-06-10   18:29:38 ET  Reply   Trace   Private Reply  


#33. To: DeaconBenjamin (#31)

When visiting my uncle in Oregon (early 70s) we had some salmon, fresh caught, grilled like steaks. I've never had the like again.

Buy WILD SALMON from a reputable store like Wegman's or Harris Teeter.

Grill it yourself basted just in a little Italian dressing.

Hot and quick do not dry out.

Tastes completely different from the farmed stuff.

If you like a milder fish, find Char !

JCHarris  posted on  2007-06-10   18:32:15 ET  Reply   Trace   Private Reply  


#34. To: JCHarris (#33)

The word Chesepiooc is an Algonquian word meaning "Great Shellfish Bay." The Bay was once known for its great seafood production, especially blue crabs, clams and oysters. The plentiful oyster harvests led to the development of the Skipjack, the State Boat of Maryland, which is the only remaining working boat type in the United States still under sail power. Today, the body of water is less productive than it used to be, because of runoff from urban areas (mostly on the western shore) and farms (especially on the eastern shore), overharvesting, and invasion of foreign species. The bay though, still yields more fish and shellfish (about 45,000 short tons or 40 000 tonnes yearly) than any other estuary in the United States.

The Bay is famous for its rockfish, also known as striped bass. Once on the verge of extinction, rockfish have made a significant comeback and are now able to be fished in strictly controlled and limited quantities.

The Bay serves as the predominate source of eel in the United States.

In 2005, local governments began debate on the introduction to certain parts of the Bay of a species of asian oyster, to revive the lagging shellfish industry.

Deteriorating environmental conditions In the 1970s, the Chesapeake Bay contained one of the planet's first identified marine dead zones, where hypoxic waters were so depleted in oxygen they were unable to support life, resulting in massive fish kills. Large algae blooms, nourished by the runoff of farm and industrial waste throughout the watershed, prevent sunlight from reaching the bottom of the Bay. The resulting loss of marine vegetation has depleted the habitat for many of the Bay's animal creatures. One particularly harmful algae is Pfiesteria piscicida, which can affect both fish and humans. The depletion of oysters due to overharvesting and damaged habitat has had a particularly harmful effect on the quality of the Bay. The Bay's oyster industry has also suffered from two diseases: MSX and dermo. Oysters serve as natural water filters, and their decline has further reduced the water quality of the Bay. Water that was once clear for metres is now so turbid that a wader may lose sight of his feet before his knees are wet.

Efforts of federal, state and local governments, working in partnership through the Chesapeake Bay Program, and the Chesapeake Bay Foundation and other nonprofit environmental groups, to restore or at least maintain the current water quality have had mixed results. One particular obstacle to cleaning up the Bay is that much of the polluting substances arise far upstream in tributaries lying within states far removed from the Bay itself.

http://www.policybers.com/Chea-to- Chri/chesapeake_bay.php

Law Enforcement Against Prohibition

IndieTX  posted on  2007-06-10   18:40:24 ET  Reply   Trace   Private Reply  


#35. To: HOUNDDAWG (#2)

Speaking as a Great Lakes denizen, the zebra mussels got here from ballast from Asian freighters. I doubt anyone picked them up here; the animal is already present in most vessels that visit the Asian region. The Great Lakes coalition just passed a law against emptying ballast in the Great Lakes region, but it's probably too late. Lake Michigan is already, due to the clams, too clean. They've found ways, temporarily, to introduce food to support the food chain, but them mussels suck up everything in their path, so the smaller fish starve, then the bigger ones do, etc.

I remember visiting the Chesapeake region when I was a kid, in the 70s, and it was almost desolate. Now it's so overgrown with McMansions and condos, it's no surprise the ecosystem is trashed. It's a pretty delicate system, and not meant to become an urban area. The coasts of Virginia and the Carolinas are being similarly stressed to death.

There's a lot of pressure here in Chicago and the northern suburbs to not only end building on the coast of Lake Michigan, but to buy back the aging skyscrapers they built in the 60s and turn the coast low-rise with much lower population density. Again, probably too late; the fish population here is constantly declining.

Mekons4  posted on  2007-06-10   18:45:55 ET  Reply   Trace   Private Reply  


#36. To: IndieTX (#34)

One particular obstacle to cleaning up the Bay is that much of the polluting substances arise far upstream in tributaries lying within states far removed from the Bay itself.

Ah yes, that terrible person that lives upstream.

Having lived on the Bay before it became so polluted and also after, I understand why it is easier to point the finger at the few that live far away.

Paving over the land near the Bay and installing millions of people with little regard for the Bay has had just a tad to do with the pollution. Dumping into and around the Bay of all manners of substances for many years has been cumulative.

Are there now more or fewer cows upstream than any time in the last 100 years? More or fewer farms?

Are there more or fewer people living in the watershed, particularly close to the Bay?

Where I once lived was vast open stretches of farmland, lots of cows, now that is gone and we have wall to wall people by the hundreds of thousands, each and everyone adding to the problems of the Bay.

Cynicom  posted on  2007-06-10   18:57:24 ET  Reply   Trace   Private Reply  


#37. To: JCHarris (#26)

I haven't a clue they are there.

You do know what causes wasting diseases, otherwise known as Transmissible Spongiform Encephalopathies (TCE), right?


You appear to be a major trouble maker...and I'm getting really pissed. - GoldiLox, 7/27/2006

FormerLurker  posted on  2007-06-10   19:10:52 ET  Reply   Trace   Private Reply  


#38. To: FormerLurker (#37)

Strangely folded proteinaceous templates.

JCHarris  posted on  2007-06-10   19:16:26 ET  Reply   Trace   Private Reply  


#39. To: JCHarris (#38)

Strangely folded proteinaceous templates.

Well most researchers are fairly certain that the cause is spiroplasmas, and that prions are just a protein shed by the spiroplasma, and not the cause itself, but an indication of infection.


You appear to be a major trouble maker...and I'm getting really pissed. - GoldiLox, 7/27/2006

FormerLurker  posted on  2007-06-10   19:29:01 ET  Reply   Trace   Private Reply  


#40. To: JCHarris (#38)

Spiroplasm a & Transmissible Spongiform Encephalopathies


You appear to be a major trouble maker...and I'm getting really pissed. - GoldiLox, 7/27/2006

FormerLurker  posted on  2007-06-10   19:29:49 ET  Reply   Trace   Private Reply  


#41. To: FormerLurker (#40)

In my humble opinion, Bastian was de-bunked a decade ago.

JCHarris  posted on  2007-06-10   19:38:31 ET  Reply   Trace   Private Reply  


#42. To: DeaconBenjamin (#31)

When visiting my uncle in Oregon (early 70s) we had some salmon, fresh caught, grilled like steaks. I've never had the like again.

I know what you mean. Same experience with three 18 inch trout caught in the Blackwater River in West Virginia, rolled in cornmeal and flour and fried over a campfire in bacon drippings.

JCHarris  posted on  2007-06-10   22:28:54 ET  Reply   Trace   Private Reply  


#43. To: JCHarris (#41)

In my humble opinion, Bastian was de-bunked a decade ago.

I guess you haven't read anything more recent on the topic then..

From Linking chronic wasting disease to scrapie by comparison of Spiroplasma mirum ribosomal DNA sequences.

Bastian FO, Dash S, Garry RF.

Department of Pathology and Laboratory Medicine, Tulane University Health Sciences Center, New Orleans, LA 70112, USA. fbastian@tulane.edu

Transmissible spongiform encephalopathies (TSE) are fatal neurodegenerative diseases of man and animals and are transmitted by a filterable pathogen whose identity is currently unresolved. Our data indicates that Spiroplasma, a wall- less bacterium, is involved in the pathogenesis of TSE. We searched for Spiroplasma ribosomal gene sequences in 10 scrapie-infected sheep brains and 10 normal sheep brains, 7 cervid samples infected with chronic wasting disease (CWD), and 7 normal cervid brains. DNA was extracted from these tissue samples and amplified by polymerase chain reaction (PCR) using primers specific for Spiroplasma-specific 16S rDNA. Specificity of the amplicon was determined by Southern blotting and DNA sequence analyses. Spiroplasma 16S rDNA was found in 8 of 10 scrapie-infected sheep brains and 6 of 7 CWD-infected tissue samples. All normal animal brain samples were negative. Spiroplasma 16S rDNA was also found in two human Creutzfeldt-Jakob diseased (CJD) brains but not in two age- matched normal human brains. DNA sequence analyses of the amplified PCR products from human and animal TSE cases revealed greater than 99% nucleotide sequence homology with Spiroplasma mirum. The presence of Spiroplasma DNA in TSE-infected tissues supports our hypothesis that Spiroplasma may be involved in the pathogenesis of these diseases.

Publication Types: Comparative Study Research Support, U.S. Gov't, P.H.S.

PMID: 15215050 [PubMed - indexed for MEDLINE]

--------------------------------------------------------------------------------


You appear to be a major trouble maker...and I'm getting really pissed. - GoldiLox, 7/27/2006

FormerLurker  posted on  2007-06-10   22:57:59 ET  Reply   Trace   Private Reply  


#44. To: FormerLurker (#43)

PMID: 16584444 [PubMed - indexed for MEDLINE]

Big problem with Bastian is that high speed (200K) centrifugation supernatants are the infectious moiety. No myco or spiroplasma is found in that fraction.

I know Tulane well and have a doctoral student there now in neurobiology...gave him his first paper in Eu+++ conjugated proteins .

Pub Med ..............................

Preparation of soluble infectious samples from scrapie-infected brain: a new tool to study the clearance of transmissible spongiform encephalopathy agents during plasma fractionation. Berardi VA, Cardone F, Valanzano A, Lu M, Pocchiari M.

Degenerative and Inflammatory Neurological Diseases Unit, Department of Cell Biology and Neurosciences, Italian National Institute of Health, Rome.

BACKGROUND: Concern about the safety of blood, blood components, and plasma-derived products with respect to prions has increased since the report of two blood-related infections of variant Creutzfeldt-Jakob disease in the United Kingdom. Efforts were directed toward the development of procedures able to remove or inactivate prions from blood components or plasma-derived products with brain fractions of transmissible spongiform encephalopathy (TSE)-infected rodents as spiking materials. These spiking materials, however, are loaded with pathological prion protein (PrP(TSE)) aggregates that are likely not associated to blood infectivity. The presence of these aggregates may invalidate these studies. STUDY DESIGN AND METHODS: Brains from 263K scrapie-infected hamsters were suspended in 10 percent phosphate-buffered saline. After low-speed centrifugation, the supernatant was collected and ultracentrifuged at 220,000 x g at 25 degrees C for 30 minutes. The high-speed supernatants (S(HS)) and pellets were collected; the proteinase-resistant PrP(TSE) was measured by Western blot and infectivity by intracerebral inoculation into weanling hamsters. RESULTS: A substantial amount of prion infectivity (more than 10(5) LD(50) per mL of a 10% suspension of brain tissues) is present in the S(HS) fraction of 263K scrapie-infected hamster brains. Concomitantly, this fraction contains none or only traces of PrP(TSE) in its aggregate form. CONCLUSION: This study describes a simple and fast protocol to prepare infectious material from 263K scrapie-infected brains that is not contaminated with PrP(TSE) aggregates. This S(HS) fraction is likely to be the most relevant material for endogenous spiking of human blood in validation experiments aimed at demonstrating procedures to remove or inactivate TSE infectious agents.

PMID: 16584444 [PubMed - indexed for MEDLINE]

JCHarris  posted on  2007-06-10   23:17:10 ET  Reply   Trace   Private Reply  


#45. To: JCHarris (#44)

Can you honesty state that injecting ONLY prions into an animal brain will CAUSE TSE? Are there any papers stating this? Is it certain that spiroplasmas were not also present?


You appear to be a major trouble maker...and I'm getting really pissed. - GoldiLox, 7/27/2006

FormerLurker  posted on  2007-06-11   0:16:40 ET  Reply   Trace   Private Reply  


#46. To: FormerLurker (#45)

Can you honesty state that injecting ONLY prions into an animal brain will CAUSE TSE? Are there any papers stating this?

Yes.

As far as I can see that is irrelevant. It goes on the same principle as an old paradigm of a "filterable particle" in earlier virology before PCR. Its as simple as that.

spiroplasma is a filterable particle of the days when you were around Phantoms ( Hey! LOL, I knew you were an old fart!!).

JCHarris  posted on  2007-06-11   3:45:15 ET  Reply   Trace   Private Reply  


#47. To: rowdee (#24)

Awe, c'mon, DAWGIE......you can do better than that.

Like this: If we someday find ourselves surrounded by bands of mutant cannibals who glow in the dark, the govt will tell the survivors that it was caused by environmentalists who insisted on mulching and recycling terrorists who hate us for our freedoms, and the people will believe it....

Much better!

More believable and will help channel that blind hatred in a more useful direction....

HOUNDDAWG  posted on  2007-06-11   15:47:21 ET  Reply   Trace   Private Reply  


#48. To: Mekons4 (#35)

Speaking as a Great Lakes denizen, the zebra mussels got here from ballast from Asian freighters. I doubt anyone picked them up here; the animal is already present in most vessels that visit the Asian region. The Great Lakes coalition just passed a law against emptying ballast in the Great Lakes region, but it's probably too late. Lake Michigan is already, due to the clams, too clean. They've found ways, temporarily, to introduce food to support the food chain, but them mussels suck up everything in their path, so the smaller fish starve, then the bigger ones do, etc.

I remember visiting the Chesapeake region when I was a kid, in the 70s, and it was almost desolate. Now it's so overgrown with McMansions and condos, it's no surprise the ecosystem is trashed. It's a pretty delicate system, and not meant to become an urban area. The coasts of Virginia and the Carolinas are being similarly stressed to death.

There's a lot of pressure here in Chicago and the northern suburbs to not only end building on the coast of Lake Michigan, but to buy back the aging skyscrapers they built in the 60s and turn the coast low-rise with much lower population density. Again, probably too late; the fish population here is constantly declining.

Thank you for an excellent post, and forgive the delayed reply.

I wanted to wait until I felt better so I could give your post due consideration.

I studied the sinking of the Edmund Fitzgerald, the specially built coast guard cutters that not only use lake water for engine cooling but are too wide to fit through the locks, both of which guarantee that the vessels are dedicated to duty on the lakes, the history of the Purple Gang's pirate fleet, and other interesting facts about the Great Lakes.

You have as much reason as I to feel sadness, because these once great treasures are suffering from the same malignant and benign neglect as my beloved Chesapeake Bay.

HOUNDDAWG  posted on  2007-06-11   16:00:04 ET  Reply   Trace   Private Reply  


#49. To: JCHarris (#29)

give me a break...give it up...just look at the harvests of anything you want to look up...that alone tells the story.

Thank you for your interesting and educational posts on this topic.

The truth is when my biologist buddies at the DE Div. of Fish and Wildlife tell me something, I believe them.

They are the only govt employees that I trust to that extent.

I see now that you're a knowledgeable professional with no axe to grind and you're quite likely entitled to that same respect.

I apologize for being obtuse. I could explain my reasons but there is no excuse, so I won't.

HOUNDDAWG  posted on  2007-06-11   16:06:54 ET  Reply   Trace   Private Reply  


#50. To: HOUNDDAWG (#49)

LOL

Hey Houndawg

We bee just talkin' an' having fun...

no agendas here and none on your side...

all is well !

Thanks

( PS did a three month graduate work stint in the bay on two separate occasions...I was a new breed then called genetic engineers.... we were strange creatures !)

Also I have friends in Reedville today and often sailed out of Reedville and near Fleetwood to Maryland , Tangier, Santa Isabella, and the Eastern Shore, when I was not sailing around Bogue Sound and Schackelford Banks in North Carolina.

Cheers !~

JCHarris  posted on  2007-06-11   16:25:37 ET  Reply   Trace   Private Reply  


#51. To: JCHarris (#50)

( PS did a three month graduate work stint in the bay on two separate occasions...I was a new breed then called genetic engineers.... we were strange creatures !)

Also I have friends in Reedville today and often sailed out of Reedville and near Fleetwood to Maryland , Tangier, Santa Isabella, and the Eastern Shore, when I was not sailing around Bogue Sound and Schackelford Banks in North Carolina.

Cheers !~

Outstanding.

HOUNDDAWG  posted on  2007-06-11   17:52:42 ET  Reply   Trace   Private Reply  


#52. To: rowdee (#23)

IIRC, there was a Swedish colony in the Delaware area, mid 1600s...would have to look back thru my genealogy notes. I have an ancestor I'm trying to find who was born in Delaware and moved to NY, and came across that bit of info. I think most people have no idea regarding that bit of history.

You bet there was!

The Old Swedes Church.

HOUNDDAWG  posted on  2007-06-12   0:09:22 ET  Reply   Trace   Private Reply  


#53. To: HOUNDDAWG (#52)

Thanks for the links, DAWG........my memory is still here! Yahhhhhhh!

I love history AND genealogy, so it makes the searching so much more interesting. :)

I see a mention of New Amsterdam in the first article. I presume that is the NY one. That is where some of my first American (as in being on US soil) ancestors were found.....that history starts in 1624 or 26 when the first one came over with the Dutch West Indies (or is it East Indies) Company went to NY and up the Hudson River where he worked as overseer for a Rennslaar (IIRC--I haven't looked at that line for a few years now).

Anyways, it wound up he and the wife and kids when down to one of the now borough areas and set up a trading post. Netje, the wife, was apparently very good at dealing with Indians. She and Garretje (Sp?) had several sons, two of which started the first beer brewery in NY, as the story goes, ca 1600s. With a name like van kouwenhoven, they shoulda done something important! LOL.

Dang it--now, I want to dig out all that 'stuff' and read it again! :)

rowdee  posted on  2007-06-12   11:13:07 ET  Reply   Trace   Private Reply  


#54. To: rowdee (#53)

yes, New Amsterdam was NY and New Amstel was DE.

Stuyvesant landed here in Old New Castle when he first set foot in the new world.

With a name like van kouwenhoven it's got to be good! ;)

HOUNDDAWG  posted on  2007-06-12   15:44:21 ET  Reply   Trace   Private Reply  


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